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Quantitative 3D Cell Culture Colony Assay Kit

Features

  • Applicable to the fields of anti-cancer drug development and cancer research, utilizing the colony-forming ability assay with anchorage-independent proliferative ability.
  • Higher accuracy and smaller variability when analyzing multiple samples than conventional colony methods
  • The product set-up is quick and simple, and no special reagent preparation is required. (Operation time is reduced to less than half.)
  • Cost is reduced to 1/10 or less compared to conventional products.

Applications

  • Anticancer Drug Discovery Screening
  • Evaluation system for induction of drug-metabolizing enzymes
  • Optimization of culture conditions for primary cancer cells
  • Optimization of cancer stem cell growth conditions

Specifications

Model Medium Composition
CAK-RPMI-8 RPMI1640  96-well microplate (1 plate)  for ３D Cell Culture Assay
〔Reagents for ３D Cell Culture〕1 bottle for each solution Solution-1 (agar): 4 ml
Solution-2 (2 × RPMI1640): 3.2 ml1
Solution-3 (1 × RPMI1640): 9 ml2
Solution-4 (1％ SDS): 2 ml
〔Reagents for assay〕 Assay-1 (WST-8):1  ml
CAK-DMEM-8 DMEM
（low glucose）		 96-well microplate (1 plate)  for ３D Cell Culture Assay
〔Reagents for ３D Cell Culture〕1 bottle for each solution Solution-1 (agar): 4 ml
Solution-2 (2 × DMEM): 3.2 ml3
Solution-3 (1 × DMEM): 9 ml4
Solution-4 (1％SDS): 2 ml
〔Reagents for assay〕 Assay-1 (WST-8): 1 ml

1,3 FCS (0.8ml) is added immediately before experiment (final: FCS 20%)

2,4 FCS (1ml) is added immediately before experiment (final: FCS 10%)

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